We are fast approaching the end of our research program, so sad! Hello, my name is Amanda Nweke and I currently work in Dr. Mothe's lab. My project is to formulate a construct to make a hypoimmunogenic stem cell using CRISPR. My research came as a result of two papers published by Harvard University and University of California San Francisco (UCSF). In both papers, hypoimmunogenic stem cells were made by knocking out HLA-A/B/C and CIITA and knocking in B2M (USCF) or PDL1, HLA-G, and CD47(Harvard). What I am currently doing now is finding if there is any MHC expression in our Gibco human induced pluripotent stem cell (hiPSC) using both PCR and a flow cytometry. Aside from being in the lab this week, we all had the opportunity to go to various networking events and seminars. Tuesday, we visited the University of California Riverside (UCR) where Dr. Glenn Hicks gave us a tour of some the research that goes on at UCR. In the first lab we visited, the PhD students spoke on their research with bumble bees and how bacteria affects the bee population. We concluded our tour with the graduate students discussing their experience as PhD candidates at UCR. I found this discussion very useful, because a lot of times some of the graduate students “don’t keep it real” to some of the undergraduate students about some of the challenges they face in graduate school. I also found the advice they gave to us useful as well. On Wednesday, we had a poster presentation seminar hosted by both Dr. Julie Jameson and Dr. Besty Read. They gave beneficial information on how to make a scientific poster and how to effectively deliver your research to an audience. They also proposed a new way of creating a poster that pinpoints the main conclusion on the poster as the forefront of the poster. On Thursday, we went to a *NSPIRE symposium poster presentation event hosted by Dr. Robert G. Iafe. In which three guest speakers who were PhD candidates at Scripps Research Institute gave a talk about their journey to a PhD as well as the research they are currently working on at Scripps. After that, there was a poster presentation given by students from California State University San Marcos.
0 Comments
Leave a Reply. |
Watch this space for weekly updates!Every week, one of our CSUSM NSF REU students will post their blurb, summarizing their week, and chronicling our program. AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
August 2023
Categories |