Almost reaching the finish line of this program and I'm truly enjoying every minute of it!
My name is Matilde Macedo and I am working in Dr. Jameson's lab which focuses on studying T cells that are involved immune diseases such as alopecia areata, and type 2 diabetes. In this lab I learn a lot of laboratory techniques such as sterilizing tools with the autoclave, working in the TC hood, and maintain cell lines. Additionally, I learn how to do epithelium preparation which consist of extracting dendric epidermal T cells (DETCs) from mice and plate them into a 96 well plate to prepare them for RNA extractions. I also learned how to do immunofluorescent staining on wounded tissues so we can visualize the t cells that are involved in wound healing. In this program I've been working on public data analyzing the RNA of people with alopecia areata using Galaxy. In this computer program I learn how to use different tools to analyze the transcriptome which includes FASTQ, Tophat, cufflinks, and cuffdiffs. My research involves in investigating if genes signatures of DETCs in patients with alopecia areata are upregulated or downregulated. This will help us picture the influence of DETCs in this disease. Overall my experience in this program had been amazing. In this week we attended the NSF GRFP webinar where we learned more about the program's application which can help recognize and financially support MS and PhD level research. To destress from our projects, we hiked the Iron Mountain to observe the beautiful new moon from up there. I am thankful that I get to have this experience which in turn will help me get a step further in reaching my career goals.
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Watch this space for weekly updates!Every week, one of our CSUSM NSF REU students will post their blurb, summarizing their week, and chronicling our program. AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
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