Hi there, my name is Hannah Hausknecht-Buss! I'm from Phoenix, Arizona, but I am a biology and Spanish major currently going into my junior year at the University of San Diego. In my free time, I enjoy playing the flute and going to the beach to relax! Week 2 went by quickly, but I had tons of fun and learned so many new things. It's an understatement to say that I am ecstatic to see what the remaining time here at the CSUSM NSF REU program has to offer!
This summer, I am participating in Dennis Kolosov's lab, where we are performing research on mosquitoes and caterpillars! More specifically, I am investigating the epithelial physiology and transcriptomic response of "yellow fever" (Aedes aegypti) mosquito larvae and adults and the prevalence of voltage gated calcium ion channels present in the osmoregulatory organs (the anal papillae and Malpighian tubules). We will be testing the presence of these channels through rapid changes in salinity and blood meal feeding and I have my fingers crossed that we discover something interesting, as there isn't any pre-existing studies that are similar to what we are delving into! To start off the week, we continued dissecting mosquito larvae so we could collect tissues (they are unbelievably small!) and prepare for an RNA extraction! We then went to check up on the "homies" (our insects) to do our weekly housekeeping and make sure they are happy and healthy. For the next couple of days, we attended a talk by Dr. Read and Dr. Sethuraman on assembling the genome and performing BlastX as well as continuing to learn UNIX coding. We also received a special talk on genomics from Dr. Mark Chaisson from USC! By the end of the week, I started dissecting adult mosquitoes, and even developed a new method for how to catch them in their cage (they are even harder to dissect than larvae, surprisingly!). Lastly, we also designed our own primers to use in PCR and created a seawater solution to use for testing rapid salinity response in our larvae. There was a ton to do outside of lab as well! My lab group and I, as well as some fellow REU friends, spent a lot of dinners together at places like Manna Korean BBQ and Burger Bench (I highly recommend <3). Our program also met up for a Taco Thursday and I always love going to these big group events so I can see and meet new people from other lab groups! To finish off our second week, some of us headed down to La Jolla and Downtown SD to see some sea lions and walk around Balboa Park! I am so blessed and grateful to be here! I have made some amazing new friends and everyone has been so kind. This summer will definitely be one for the books!
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Watch this space for weekly updates!Every week, one of our CSUSM NSF REU students will post their blurb, summarizing their week, and chronicling our program. AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
August 2023
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